Addition of zeolite in EGSB reactor for the treatment of swine wastewater

Main Article Content

Tania Pérez-Pérez
Ileana Pereda-Reyes
Gleyce Teixeira Correia
Eloisa Pozzi
Wu Hong Kwong
Deny Oliva-Merencio
Marcelo Zaiat

Abstract

The effect of zeolite addition in an expanded granular sludge bed anaerobic reactor (EGSB) for the treatment of swine wastewater at high organic loading rates (OLR) was evaluated. The results showed that the presence of the zeolite did not affect the hydrodynamics of the EGSB, adjusting the model of tanks in series. Two reactors (R1 and R2) were operated with effective volume of 3.04 L at 30C. Reactor 1 was operated only with granular sludge while 120 g of zeolite (5 cm of static bed) were added in R2. Theoretical hydraulic retention time (HRT) was 12 hours, obtained with an influent flow rate of 4.0 mL/min. Firstly; an organic load shock (OLS) strategy with constant up-flow velocity (vup) of 6 m/h for 180 days was evaluated. The evolution of pH, Total Kjeldahl Nitrogen (TKN), Chemical Oxygen Demand (COD) and volatile fatty acids (VFAs) was monitored during the anaerobic digestion process with OSL and the increase of the organic loading rate (OLR). In addition, the microbial composition and the changes in bacterial and archaeal community structures were assessed. The reactor with zeolite addition showed a higher stability at high OLR reaching efficiency of 80% at 32 kgDQO/m3d. Subsequently, gradual increase of both OLR and the up-flow velocity to 10 m/h for a period of 255 days was evaluated. In this study, a trend never before reported was observed, increasing its efficiency to 90% and achieving a higher rate of organic degradation. It can be ensured that the addition of 40 g/L zeolite to the EGSB allowed the higher stability of the system with robustness when varying the operational conditions. Moreover, changes in microbial diversity were observed.

Article Details

How to Cite
Pérez-Pérez, T., Pereda-Reyes, I., Teixeira Correia, G., Pozzi, E., Hong Kwong, W., Oliva-Merencio, D. and Zaiat, M. (2019) “Addition of zeolite in EGSB reactor for the treatment of swine wastewater”, INFOMIN, 11. Available at: https://infomin.edicionescervantes.com/index.php/i/article/view/120 (Accessed: 11 August 2026).
Section
Artículos Originales

References

Abouelenien, F.; Namba, Y.; Kosseva, M.R.; Nishio, N.; Nakashimada, Y. 2014. Enhancement of methane production from co-digestion of chicken manure with agricultural wastes. Bioresource Technology, Vol.159: 80-87.

Ambuchi, J.J.; Liu, J.; Wang, H.; Shan, L.; Zhou, X.; Mohammed, M.O.; Feng, Y. 2016. Microbial community structural analysis of an expanded granular sludge bed (EGSB) reactor for beet sugar industrial wastewater (BSIW) treatment. Applied Microbiology and Biotechnology, Vol.100: 4651-4661.

Bergmann, B.A.; Cheng, J.; Classen, J.; Stomp, A. 2000. In vitro selection of duckweed geographical isolates for potential use in swine lagoon effluent renovation. Bioresource Technology , Vol.73, (No.1): 13-20.

Chernicharo, C. A. L. 2007. Anaerobic reactors. Biological Wastewater Treatment Series, Vol.4,London: IWA Publishing, 175 p., ISBN: 978-1-78040-211-6, Available: Available: https://www.amazon.com/Anaerobic-Reactors-Biological-Wastewater-Treatment/dp/1843391643 , [Consultado: Enero 10, 2015].

Chou, H. H.; Huang, J.Sh.; Chen, Sh.K.; Lee, M.Ch.. 2011. Process kinetics of an expanded granular sludge bed reactor treating sulfate-containing wastewater. Chemical Engineering Journal, Vol.170: 233-240.

Climent, M.; I. Ferrer; M Baeza.; Artola, A.; Vázquez, F.; Font, X. 2007. Effects of thermal and mechanical pretreatments on secondary sludge on biogas production under thermophylic conditions. Chemical Engineering Journal , Vol.133, (No.1-3): 335-342.

Delforno, T.P.; Moura, A.G.L.; Okada, D.Y.; Varesche, M.B.A. 2014. Effect of biomass adaptation to the degradation of anionic surfactants in laundry wastewater using EGSB reactors. Bioresource Technology , Vol. 154: 114-121.

Di Rienzo, J.A.; Balzarini, M.G.; Casanoves, F.; González, L.; Tablada, M.; Robledo. C.W. 2012. InfoStat. [Windows], Universidad Nacional de Córdoba, Argentina: Grupo InfoStat, Available: Available: http://www.infostat.com.ar/ . [Consultado: Abril 19, 2017].

Fang, C.; Boe, K.; Angelidaki, I. 2011a. Biogas production from potato-juice, a by-product from potato-starch processing, in upflow anaerobic sludge blanket (UASB) and expanded granular sludge bed (EGSB) reactors. Bioresource Technology , Vol.102: 5734-5741.

Fang, C Thong; Boe, K.; Angelidaki, I. 2011b. Comparison of UASB and EGSB reactors performance, for treatment of raw and deoiled palm oil mill effluent (POME). Journal of Hazardous Materials, Vol.189: 229-234.

Fernández-López, M.; Puig-Gamero, M.; López-González, D.; Ávalos-Ramirez, A.; Valverde, J.; Sánchez-Silva, L. 2015. Life cycle assessment of swine and dairy manure: pyrolysis and combustion processes. Bioresource Technology , Vol.182: 184-192.

Ghorbanian, M.; Lupitskyy, R.M.; Satyavolu, J.V.; Berson, R.E. 2014. Impact of Hydraulic Retention Time at Constant Organic Loading Rate in a Two-Stage Expanded Granular Sludge Bed Reactor. Environmental Engineering Science, 31(6): 317-323, ISSN: 1557-9018, DOI:10.1089/ees.2013.0501.

Jiménez, J. 2015. Adición de paja de arroz y arcillas residuales a la digestión anaerobia de estiércol porcino. Efecto sobre la comunidad procariota productora de metano. Tesis presentada en opción al grado científico de Doctor en Ciencias Técnicas, Universidad de La Habana, La Habana, Cuba.

Kato M. T.; Field, J. A.; Versteeg, P.; Lettinga, G. 1994. Feasibility of expanded granular sludge bed reactors for the anaerobic treatment of low-strength soluble wastewaters. Biotechnology and Bioengineering, Vol.44, (No.4): 469-479.

Lafita, C.; Penya-Roja, J.M.; Gabaldon, C. 2015. Anaerobic removal of 1-methoxy-2 propanol under ambient temperature in an EGSB reactor. Bioprocess Biosystem Engineering, Vol.38, (No.11): 2137-2146.

Leven, L.; Eriksson, A.R.B.; Schnürer, A. 2007. Effect of process temperature on bacterial and archaeal communities in two methanogenic bioreactors treating organic household waste. FEMS Microbiology Ecology, Vol.59, (No.3): 683-693.

Li, W.; Su, C.; Liu, X.; Zhang, L. 2014. Influence of the organic loading rate on the performance and the granular sludge characteristics of an EGSB reactor used for treating traditional Chinese medicine wastewater. Environmental Science and Pollution Research, Vol.21, (No.13): 8167- 8175.

Liao, R.; Y. Li; X. Yu; P. Shi; Z. Wang; K. Shen; Q. Shi; Y. Miao; W. Li; A. Li. 2014. Performance and microbial diversity of an expanded granular sludge bed reactor for high sulfate and nitrate waste brine treatment. Journal of Environmental Sciences, Vol. 26, (No.4): 717-725.

Liao, R .; K. Shen ; Li, A.M.; Shi, P.; Li, Y.; Shi, Q.; Wang, Zh. 2013. High-nitrate wastewater treatment in an expanded granular sludge bed reactor and microbial diversity using 454 pyrosequencing analysis. Bioresource Technology , Vol.134: 190-197.

Liu, J.; J. Hu; J. Zhong; J. Luo; A. Zhao; F. Liu; R. Hong; G. Qian; Z.P. Xu. 2011. The effect of calcium on the treatment of fresh leachate in an expanded granular sludge bed bioreactor. Bioresource Technology , Vol.102: 5466-5472.

Liu, J .; J. Luo ; J. ZhouL; Q. Liu; G. Qian ; Z.P. Xu . 2012. Inhibitory effect of high-strength ammonia nitrogen on bio-treatment of landfill leachate using EGSB reactor under mesophilic and atmospheric conditions. Bioresource Technology , Vol.113: 239-243.

Liu, J .; J. Zhong ; Y. Wang; Q. Liu ; G. Qian ; L. Zhong; R. Guo; P. Zhang; Z. P. Xu. 2010. Effective bio-treatment of fresh leachate from pretreated municipal solid waste in an expanded granular sludge bed bioreactor. Bioresource Technology , Vol.101: 1447-1452.

Martín, G.M. 2017. Desde el 2010 Cuba no importa carne de cerdo. Juventud Rebelde, 30 January, La Habana, Cuba, ISSN: 1563-8340, Available: Available: http://www.juventudrebelde.cu/cuba/2017-01-30/desde-el-2010-cuba-no-importa-carne-de-cerdo , [Consulted: October 14, 2017].

McGlone, J.J. 2013. The future of pork production in the world: towards sustainable, welfare-positive systems. Animals, Vol.3, (No.2): 401-415.

McMahon, K. D.; Stroot, P. G.; Mackie, R. I.; Raskin, L. 2001. Anaerobic codigestion of municipal solid waste and biosolids under various mixing conditions - II: Microbial population dynamics. Water Research, Vol.35, (No.7): 1817-1827.

Nicolella, C.; Loosdrecht, M.C.M.; van Heijnen, J.J. 2000. Wastewater treatment with particulate biofilm reactors. Journal of Biotechnology, Vol.80, (No.1): 1-33.

Pereira, E. L.; C.M.M. Campos; F. Motteran. 2009. Efeitos do pH, acidez e alcalinidade na microbiota de um reator anaeróbio de manta de lodo (UASB) tratando efluentes de suinocultura. Revista Ambiente & Água, Vol.4, (No.3): 157-168.

Puñal, A.; Brauchi, S.; Reyes, J.G.; Chamy, R. 2003. Dynamics of extracellular polymeric substances in UASB and EGSB reactors treating medium and low concentrated wastewaters. Water Science and Technology, Vol.48, (No.6): 41- 49.

Ripley, L.E.; Boyle, W.C.; Converse, J.C. 1986. Improved alkalimetric monitoring for anaerobic-digestion of high strength wastes. Journal Water Pollution Control Federation, Vol.58, (No.5): 406-411.

Seghezzo, L.; Zeeman, G.; Lier, J.B. Van; Hamelers, H.V.M.; Lettinga, G. 1998. A review: the anaerobic treatment of sewage in UASB and EGSB reactors. Bioresource Technology , Vol.65, (No.3): 175-190.

Siegert, I.; Banks, C. 2005. The effect of volatile fatty acid additions on the anaerobic digestion of cellulose and glucose in batch reactors. Process Biochemistry, Vol.40, (No.11): 3412-3418.

Xu, J.; Adair, Ch.W.; Deshusses, M.A. 2016. Performance Evaluation of a Full-Scale Innovative Swine Waste-to-Energy System. Bioresource Technology , Vol.216: 494-502.

Yu, H.; Chen, Ch.; Ma, J.; Xu, X.; Fan, R.; Wang, A. 2014. Microbial community functional structure in response to micro-aerobic conditions in sulfate-reducing sulfur-producing bioreactor. Journal of Environmental Sciences , Vol.26, (No.5): 1099-1107.

Zheng, H.; Li, D.; Stanislaus, M. S.; Zhang, N.; Zhu, Q.; Hu, X.; Yan, Y. 2015. Development of bio-zeolite fixed bed bioreactor for mitigating ammonia inhibition of anaerobic digestion with extremely high ammonium concentration livestock waste. Chemical Engineering Journal , Vol.280: 106-114

Ziganshina, E.E.; Belostotskiy, D.E.; Ilinskaya, O.N.; Boulygina, E.A.; Grigoryeva, T.V.; Ziganshin, A.M. 2015. Effect of the Organic Loading Rate Increase and the Presence of Zeolite on Microbial Community Composition and Process Stability During Anaerobic Digestion of Chicken Wastes. Environmental Microbiology, Vol.70, (No.4): 948-970.